NOTCH1基因敲除质粒

pLenti-NOTCH1-sgRNA (NOTCH1基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的NOTCH1基因敲除的质粒(L29135 pLenti-NOTCH1-sgRNA)、慢病毒(L29136 NOTCH1 Knockout Lentivirus)、HEK293T细胞(L29137 NOTCH1 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L29138 NOTCH1 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L29139 NOTCH1 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

NOTCH1基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	NOTCH1	4851	BC013208	NM_017617

 

About the gene
Official Symbol	NOTCH1
Previous Symbol	TAN1
Official Full Name	notch receptor 1
Synonyms	hN1; AOS5; TAN1; AOVD1
Location	9q34.3
Gene Type	protein_coding
Uniprot ID	P46531
Pathway/Library	others
Gene Summary	This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.