HHIPL2基因敲除质粒

pLenti-HHIPL2-sgRNA (HHIPL2基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的HHIPL2基因敲除的质粒(L13015 pLenti-HHIPL2-sgRNA)、慢病毒(L13016 HHIPL2 Knockout Lentivirus)、HEK293T细胞(L13017 HHIPL2 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L13018 HHIPL2 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L13019 HHIPL2 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

HHIPL2基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	HHIPL2	79802	BC015201	NM_024746

 

About the gene
Official Symbol	HHIPL2
Previous Symbol	KIAA1822L
Official Full Name	HHIP like 2
Synonyms	FLJ13840
Location	1q41
Gene Type	protein-coding gene
Uniprot ID	Q6UWX4
Pathway/Library	Lung Cancer Growth Related Genes Library
Gene Summary	FAC and FeCi exposure modulated cellular iron status with a decrease in TFRC mRNA level and an increase in intracellular ferritin level. FAC increased ROS level and caspase 3 activity. Ferroportin, HFE and TFR2 mRNAs were expressed in MG-63 cells under basal conditions. The level of ferroportin mRNA was increased by iron, whereas HFE mRNA level was decreased. The level of mRNA alpha 1 collagen type I chain, osteocalcin and the transcriptional factor RUNX2 were decreased by iron. Transcriptomic analysis revealed that the mRNA level of HedgeHog Interacting Protein Like-2 (HHIPL-2) gene, encoding an inhibitor of the hedgehog signaling pathway, was decreased in the presence of FAC. Specific inhibition of HHIPL-2 expression decreased osteoblast marker mRNA levels. Purmorphamine, hedgehog pathway activator, increased the mRNA level of GLI1, a target gene for the hedgehog pathway, and decreased osteoblast marker levels. GLI1 mRNA level was increased under iron exposure.