C19orf48基因敲除质粒

pLenti-C19orf48-sgRNA (C19orf48基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的C19orf48基因敲除的质粒(L12575 pLenti-C19orf48-sgRNA)、慢病毒(L12576 C19orf48 Knockout Lentivirus)、HEK293T细胞(L12577 C19orf48 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L12578 C19orf48 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L12579 C19orf48 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

C19orf48基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	C19orf48	84798	BC006151	NM_032712

 

About the gene
Official Symbol	C19orf48
Previous Symbol	-
Official Full Name	chromosome 19 open reading frame 48
Synonyms	MGC13170; BC006151
Location	19q13.33
Gene Type	protein-coding gene
Uniprot ID	Q6RUI8
Pathway/Library	Lung Cancer Growth Related Genes Library
Gene Summary	C19orf48 as the gene encoding a novel minor H antigen presented by HLA-A*0201 and recognized by CTL clones isolated from two patients with metastatic RCC who were treated with nonmyeloablative HLA-identical allogeneic HCT. The C19orf48 gene, first identified by the Mammalian Gene Collection Program, was subsequently cloned from a cisplatin-resistant human lung adenocarcinoma cell line. Sequence analysis of C19orf48 transcripts predicted a 117-residue translation product encoded by the largest ORF that is located in both the NM_199250 and NM_199249 transcripts. The studies of T cell recognition of C19orf48 provide direct evidence for a translation product derived from a novel ORF in the +2 frame of NM_199250 that encodes a predicted polypeptide of only 48 residues. Although differential splicing of 5 exons result in the alternative C19orf48-derived transcripts NM_199249 or NM_199250 that are discordant for the ORF encoding the antigenic 48-residue protein, our analyses of transcript expression by both northern blot and Q-PCR demonstrate that the NM_199250 transcript encoding the antigenic 48 residue polypeptide is the dominant transcript, with expression levels that are 100- to 600-fold greater than the expression levels of NM_199249. Discovery of the C19orf48-encoded minor H antigen within an unrecognized ORF adds to a growing body of data demonstrating that minor H peptides can be derived from surprisingly short protein species encoded by unconventional or occult ORFs. The C19orf48 genomic locus lies between the androgen-regulated testicular acid phosphatase (ACPT) gene and the androgen-regulated kallikrein gene cluster on chromosome 19q.