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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
anti-DNA-POD (1: 5; 1: 10) overnight at 4°C. 7. Wash in PBS (3x5 min). 8. Demonstration of peroxidase activity in a Petri dish: dissolve a DAB tablet (10 mg) in 40 ml of 0.1M citrate acid-sodium citrate buffer (pH 5.5), add 5 ml of H2
this step if you want the highest level of stable isotope labeling, and then continue to Step 6. To produce purified [13 C3 15 N]-CoASH, continue to Step 27. testing of sIlec labeling (6) Short-chain acyl-CoA acid extraction
-quadrupole mass spectrometers are most commonly used to obtain amino acid sequences. In the first stage of analysis, the machine is operated in MS scan mode and all ions above a certain m /z ratio are transmitted to the third quadrupole for mass analysis (Fig
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