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文献和实验. Here, we describe a novel use of acridine orange to visualize lysosomes and discriminate cells with healthy lysosomes from cells with damaged lysosomes in two different types of mammalian cells: fibroblasts and macrophages. This method allows assessment of lysosomal
of nonisotopic probes synthesized with Ambion's MAXIscript™ and BrightStar™ Psoralen-Biotin Kits. The alternative to UV shadowing is staining with ethidium bromide or acridine orange and requires subsequent extraction of the dye. The detection limit of UV
purification of nonisotopic probes synthesized with Ambion's MAXIscript™ and BrightStar™ Psoralen-Biotin Kits. The alternative to UV shadowing is staining with ethidium bromide or acridine orange and requires subsequent extraction of the dye. The detection
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