• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies

        互联网

        694
        Acridine orange (AO) is the most notable member of the acridine fluorescent dye family. Because of its versatility and diverse biological applications, it has been widely used in basic and clinical research fields (1 10 ). For flow cytometric application, AO offers distinct advantages over other fluorochromes by simultaneously binding to DNA and RNA in individual cells. RNA quantitation augments conventional DNA analysis by indirectly assessing the biological activity of tumor populations as it relates to the transcriptional activity, cell proliferation, and differentiation status of cells (11 ). However, flow cytometric application of this dye remains limited, because of concerns regarding procedure tediousness and its notoriety for contaminating tubing of flow cytometers. These difficulties can be overcome and successful results can be obtained by adherence to strict staining protocols and careful maintenance of instrumentation.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序