Relaxed plasmid DNA - 0.050 mg

QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒

      ♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒DNA

Purification of Supercoiled Plasmid DNA

of transcription and replication, respectively. These supercoils must be relaxed in order for transcription and replication to continue unaffected, as outlined in preceding chapters. The DNA topoisomerases play the central role in relaxing this supercoiling (1 -4 ).

Plasmid DNA minipreps

buffer:10 mM Tris HCl pH8;1 mM EDTA. RNase solution: 50 ul of 10 mg/ml RNase;5 ml TE. 2.Alternative ammonium acetate method This is a shorter version of the above.If the DNA is not clean enough. add a step at the end.Pellet and resuspend 1.5 ml of o