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- 2025年07月07日
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文献和实验Liquid Chromatography‐Mass Spectrometry Analysis of DNA Polymerase Reaction Products
Boosalis, M.S., Petruska, J., and Goodman, M.F. 1987. DNA polymerase insertion fidelity: Gel assay for site‐specific kinetics. J. Biol. Chem. 262:14689‐14696.
;6.After the third wash the bound beads were eluted using elution buffer II, incubated at 65°C for 10 minutes and separated using the magnetic bead separator;7.The final ~100µl elute is then assessed for promoter specific DNA and/or RNA by PCR and RT-PCR respectively
Bst-catalyzed radiolabeled DNA sequencing
, nucleotide extension mix, either [a-32-P]dATP or [a-35-S]dATP, and Bst polymerase) are added. Reactions are performed as described above for single-stranded sequencing. Protocol For single-stranded DNA sequencing: 1. Prepare the following
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