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100 assays
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文献和实验, A.R., Barnum, J.E., Harmon, L.S., DeLuca, J.G., and Nichols, W.W. 1994. Rapid DNA degradation in primary rat hepatocytes treated with diverse cytotoxic chemicals: Analysis by pulsed‐field gel electrophoresis and implications for alkaline elution assays
DNA Fragmentation Assays for Apoptosis
Protocol II: DNA Fragmentation Assay via Dipheylamine In 24-wells plate, incubate 5 X 106 targets with desired number of effectors. After incubation, transfer the samples to 15ml tubes, centrifuge for 30 s at 1500g, and resuspend in 5ml
DNA Fragmentation Assays for Apoptosis Protocol
Protocol I: Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of effectors (105 target cells per well). After incubation, collect the cell sample in 1.5 ml eppendorf
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