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文献和实验DNA Topoisomerase II-Catalyzed DNA Decatenation
catenated DNA duplexes found at the end of replication. The decatenation of DNA molecules is a topoisomerase II-specific reaction, and is a convenient assay for measuring topoisomerase II activity in vitro (1 ).
Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
DNA gyrase and DNA topoisomerase (topo) IV are the bacterial targets of coumarin and quinolone antimicrobial agents. Widespread resistance to clinically important antibiotics such as beta-lactams and macrolides has stimulated the development
Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay
-specific modification interference, phosphate modification interference, and site-specific photocrosslinking. This chapter focuses on the use of the electrophoretic mobility shift assay (EMSA) to study topoisomerase binding to DNA.
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