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文献和实验All of the basic functional assays of complement activity utilize erythrocytes as targets (1 ). For classical pathway assays, the favored target is the antibody-sensitized sheep erythrocyte. For alternative pathway assays, unsensitized rabbit
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
methods that can accurately determine the genotypes and phenotypes of human complement components C4A and C4B. Specifically, they allow investigators to determine how many C4 genes are present in a diploid genome of a human subject and to quantify
4.1 ), using 15% acrylamide (15%T:5%C) in 2.5 M urea and 0.9 M acetic acid. Set up the gel in the electrophoresis unit and run the gel at 2 mA/gel for 2 hours with no sample, using 0.9 M acetic acid for the running buffer. Dissolve
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