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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
to evaluate the cellular capability to cope with exogenous stressors, we investigated stress response profiles in cultured human fibroblasts exposed to short-term heat shock or oxidative stress. Quantitative PCR analysis as well as Western blotting
Quantitation of Heat-Shock Proteins in Clinical Samples Using Mass Spectrometry
capabilities of mass spectrometers. The highly expressed proteins represent potential biomarkers that could aid in diagnosis, therapy, or drug development. Because the proteome is so complex, there is no one standard method for preparing protein samples
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