S. aureus RNA polymerase assay

Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit

into the pCRII vector. This plasmid was then used to generate RNA probes for use in a ribonuclease protection assay. Although much of the description of the ligation and transformation procedures is based on directions supplied by Invitrogen, some steps differ

The Polymerase Chain Reaction (PCR)

Topics　　• What is the Polymerase Chain Reaction?　　• History and (pre-history) of PCR　　• How PCR works　　• Optimising PCR　　• Fidelity, errors and cloning　　• PCR primer design　　• Applications of PCR What is the Polymerase Chain Reaction?　　• It’s

Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)

studies of several recombinant HCV nonstructural proteins have been initiated, our knowledge of the HCV NS5B polymerase that encodes an RNA-dependent RNA polymerase (RDRP) is rudimentary (4 –8 ). RNA-dependent RNA polymerases represent a class of viral