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文献和实验Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
into the pCRII vector. This plasmid was then used to generate RNA probes for use in a ribonuclease protection assay. Although much of the description of the ligation and transformation procedures is based on directions supplied by Invitrogen, some steps differ
The Polymerase Chain Reaction (PCR)
Topics • What is the Polymerase Chain Reaction? • History and (pre-history) of PCR • How PCR works • Optimising PCR • Fidelity, errors and cloning • PCR primer design • Applications of PCR What is the Polymerase Chain Reaction? • It’s
Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)
studies of several recombinant HCV nonstructural proteins have been initiated, our knowledge of the HCV NS5B polymerase that encodes an RNA-dependent RNA polymerase (RDRP) is rudimentary (4 –8 ). RNA-dependent RNA polymerases represent a class of viral
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