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- 2025年07月16日
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文献和实验Methods to Identify and Characterize Inhibitors of Bacterial RNA Polymerase
of multiple different classes of natural products that selectively target bacterial RNA polymerase, and the identification of a limited number of synthetic compound inhibitors, only agents of the rifamycin class have been developed and approved for human
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
into the pCRII vector. This plasmid was then used to generate RNA probes for use in a ribonuclease protection assay. Although much of the description of the ligation and transformation procedures is based on directions supplied by Invitrogen, some steps differ
The Polymerase Chain Reaction (PCR)
by the integrity of the starting target DNA — > 50 kb. Can I PCR Amplify RNA? • Not directly — the DNA polymerase requires a DNA template and will not copy RNA. • mRNA can first be copied into cDNA using reverse transcriptase. • cDNA is a template for PCR —
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