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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
restriction enzyme (e.g., EcoRI). Third, intramolecular ligation after cross-linking and digestion will lead to a fraction of the DNA occurring as circular DNA that cannot be cloned; this might further reduce the number of clones in such an assay. Finally
Cloning vector to 1ml of the Taq-amplified PCR product2. Incubate for 5 minutes on your bench top3. Transform One Shot® Competent E. coli.Topo平端克隆方法和长PCR片断克隆法（Zero Blunt Topo PCR Cloning Kit）（Topo XL PCR Cloning Kit）因为越来越多高确限度热稳定酶如Pfx、Pfu被用于PCR扩增，使到PCR
2. Incubate for 5 minutes on your bench top 3. Transform One Shot® Competent E. coli. Topo平端克隆 方法和长PCR 片断克隆 法（Zero Blunt Topo
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