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文献和实验Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I
for the major DNA-relaxing activity of the cell and is essential in an otherwise wild-type E. coli (3 ). E. coli and human topo I (htopoI) both catalyze the relaxation of negatively supercoiled DNA. However, they differ in the details of the reactions they catalyze
Overexpression and Purification of Bacterial Topoisomerase IV
Escherichia coli topoisomerase IV (topo IV) was discovered by Kato et al. (1 ), who showed that the predicted open reading frames from the parC and parE genes encoded proteins with a high degree of amino acid similarity to gyrA and gyrB
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol
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