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- Profoldin
- T2DD-96KE
- 2025年07月13日
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96 assays
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文献和实验E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol
96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
Let sit at Rt for >15’ Suck off the medium of the 96 wells and add 100 ul of mixture (step 5e) by multichanel pipette. Let sit in incubator for >1.5 hours. Add 100 ul of D20F and let it grow for two days. Dual luciferase assay: Reagents:
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
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