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- Profoldin
- T2DD-96KS
- 2025年07月12日
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文献和实验Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities
on DNA supercoiling by S. pneumoniae gyrase, DNA decatenation by S. pneumoniae topo IV, and DNA cleavage by both enzymes. These approaches provide mechanistic insight on inhibitor action and allow identification of dual gyrase/topo IV targeting agents
96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
Dual luciferase kit is in the bottom shelf of the freezer containing Mark and Greg’s stuff. The manual can be found in my bench drawer. 1. Suck off the medium from 96 well plates and wash with PBS once. Add 50 ul of 1Xpassive lysis buffer
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of interest. The immunoprecipitated DNA can then be amplified using primers spanning the restriction enzyme site found to be involved in the 3C ligation with other partner(s) in each of the 6C clones. In early attempts of the 6C assay (Tiwari et al. 2008), screening
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