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- Cedarlane
- CLY800-03AF-100UG
- 2025年07月15日
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文献和实验Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Media (Catalog # AR005 , AR007 ). The protocol below has been used with the BG01V line
Fibroblast Cell Systems Instructions for Use
below, conveniently packaged as single-use aliquots called SingleQuots® (amounts indicate concentration of each SingleQuot ®) 1 mg/ml hFGF (human recombinant Fibroblast Growth Factor) (CC-4065) 0.5 ml 5 mg/ml Insulin (CC-4021) 0.5 ml 50 mg/ml Gentamicin, 50
Craig, C.G., Tropepe, V., Morshead, C.M., Reynolds, B.A., Weiss, S., and van der Kooy, D. 1996. In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain. J
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