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文献和实验The CRE Luc Mouse Model for Bioimaging Ligand Activation of G Protein-Coupled Receptors
from a luciferase reporter have been used extensively in vitro with high-throughput screens (HTS) of large chemical compound libraries. We have generated a transgenic mouse model (CRE luc) with a luciferase reporter under the control of a synthetic promoter
Using AutoDock for Ligand‐Receptor Docking
Figure 8.14.15 Indinavir docked into a pocket in HIV‐1. Here the molecular surface has been colored using DG colors, the ligand is displayed as ball‐and‐sticks, and the complex has been rotated using the middle mouse button
ELISA-based assay for scatchard analysis of ligand-receptor interactions
of the semi-intact cell endocytosis assays. After binding of the biotinylated ligand to its receptors on the semi-intact cell membranes, a rapid centrifugation step separates the membranes from unbound ligand. Bound ligand is subsequently released by detergent
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