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文献和实验Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members
-2 homologs are cumbersome. Here, we describe highly reproducible, simple methods that we have developed to assay the activities of adenovirus E1B 19 kDa, Bcl-2, and various pro-apoptotic interacting partners.
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
) (1 ). This 36-kDa protein is remarkably stereo selective in recognizing the isomer of retinaldehyde mvolved in vision, and carries 11-cis -retinaldehyde or 11-cis -retinol as physiological ligands (2 ). Notably, retinoid bound to CRALBP is less susceptible
Phage‐Based Expression Cloning to Identify Interacting Proteins
expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins
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