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- CLX401APC
- 2025年07月14日
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Intracellular Immunofluorescent Staining for Flow Cytometry
Right: Mouse splenocytes were stimulated for 3 days with IL-2. Cells were surface stained with APC CD49d (DX5) (Cat. No. 17-5971 ) and subsequently stained intracellularly with PE GB11. Human IL-17 (eBio64CAP17): Human
血清） （must be the same species as primary antibody）。This control is easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同，应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat CD11b，clone OX-42 purified IgG
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