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文献和实验Rinse with PSA 3x 0.3% hydrogen peroxide in PBS, 30min. Then rinse in PBS 3x 5% normal goad serum in PBS at RT for 1 hour. Rabbit anti-Human Factor VIII Related Antigen diluted to 1:500 with 1% NGS in PBS, incubated
-human immunoglobulin (Ig)A, anti-human IgM and anti-human IgE were developed for the simultaneous capture of total IgG, IgA, IgM and IgE (IgGAME) antibodies from human or non-human primate serum. The fraction of antigen-specific antibodies captured
thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
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