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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
precipitate which develops at intermediate rates. The color can be distinguished from the purple color derived from X-Phos + NBT. Adding tetrazolium red or blue makes the product look more purple (less pink). Salmon-phos (Biosynth International) results
Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds
I), as well as high alkaline phosphatase (Alk Phos) activity and calcium phosphate mineralization. Interestingly, in 3D self-assembling peptide scaffold cultures, the frequency of appearance of embryonic stem-cell-like colonies was substantially enhanced, suggesting
Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5RACE
of the message. After cDNA synthesis, the first-strand product is purified from unincorporated dNTPs and ALK1. Terminal deoxynucleotidyl transferase is used to add homopolymeric tails to the 3′ ends of the cDNA. Tailed cDNA is then amplified by PCR using a nested
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