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文献和实验Adenoviral Gene Expression and Replication in Human Tumor Explant Models
conditions, primary human tumor tissue remained viable for up to 48 h, enabling evaluation of viral mutants in tissue with intact morphology. This assay may have great utility to investigate novel viral mutants and to identify treatment sensitive cancers
Construction of Recombinant Human Cytomegalovirus
viruses may also have applications in biotechnology and gene therapy. Such studies are well advanced for herpes simplex virus (HSV), a relative of human cytomegalovirus (HCMV), but have been relatively limited for HCMV itself.
Production and Characterization of Recombinant Human and Murine TNF
Here we describe the methods for the expression of human and murine soluble tumor necrosis factor (hTNF and mTNF) in Escherichia coli cells and for their extraction, purification, and characterization. The expression and purification procedure
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