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文献和实验Studies of the Ubiquitin Proteasome System
can, in general, be covalently modified by polypeptides, resulting in alterations in their fate and function. The first?identified and most well studied of these modifying polypeptides is ubiquitin. Although targeting for proteasomal degradation is the best
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Sonicate the bacterial suspension on ice, in short 10-sec bursts alternated with 10 sec resting on ice. Three cycles of sonication are usually sufficient. Oversonication can result in degradation and denaturation of the fusion
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
the function and substrate specificity of DUBs. Methods described will include: fluorescence assay using ubiquitin-amidomethylcoumarin (AMC); a high-performance liquid chromatography assay using ubiquitin ethyl ester or ubiquitin fusion peptides as model
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