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文献和实验The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
Materials Ketamine (100 mg/ml, e.g., Bela‐Pharm GmbH & Co., http://www.bela‐pharm.com/) Xylazine (20 mg/ml; cp‐pharma
ORDERING INFORMATION Catalog Number: HAF007 Lot Number: FIM06 Size: 1 mL Formulation: 0.2 μm filtered solution in PBS with stabilizers Storage: 2 - 8° C Specificity: mouse IgG1, mouse IgG2A, mouse IgG2B, mouse IgG3 heavy and light chains Immunogen
Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
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