Recombinant Human H3 Histone F

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

1a).Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein expressing plasmid

In Vitro Histone Demethylase Assays

and further understanding the regulation of these enzymes will provide significant insights into fundamental mechanisms of many biological processes and human diseases. This chapter describes different assay conditions and detection methods for different family of histone

Sulfyhydryl-Reactive Site-Directed Cross-Linking as a Method for Probing the Tetrameric Structure of Histones H3 and H4

of both recombinant and native chromatin complexes consisting either of histone subunits alone or in association with accessory proteins, in this case histone chaperones. The approaches described may be generally applicable for monitoring the interactions of a diverse