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文献和实验Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
reporter genes can be used to measure transcription activity, such as chloramphenicol acetyl transferase (CAT), luciferase, (β-galactosidase, and human growth hormone (7 ). To examine the transcription enhancement activity of a putative promoter/enhancer
Introduction of human 14-3-3 proteins
terminus of GPIb-alpha,as indicated by antibody inhibition and direct binding of 14-3-3zeta to synthetic GPIb-alpha cytoplasmic domain peptides.The 14-3-3zeta binds to recombinant wild type GPIb-IX but not to the GPIb-alpha mutants lacking C-terminal
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
21 have been commonly used for expression of recombinant proteins. Inoculate 1 liter of LB containing antibiotic selection with the 5-ml overnight culture from step 1. Grow at 37°C with shaking to an OD
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