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文献和实验Human Peripheral Blood Mononuclear Cell Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained from a blood bank). Platelets, red blood cells, and neutrophils are largely depleted
Construction of Recombinant Human Cytomegalovirus
The use of reverse genetics to generate recombinant viruses allows the researcher to investigate the exact functional significance of particular viral genes during the virus life cycle, by means of their deletion or modification in the viral
A general protocol for staining cell for cytometry analysis
according to cell type, and stage of apoptosis. In some cases, investigators may also need to reduce the number of cells to 0.5 x 10 5 /100 µl and still add 5-15 µg of recombinant Annexin V to obtain optimal results. 4. Gently mix the cells
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