Recombinant Human EGF

DESCRIPTION

Recombinant Human Interferon Alpha-2b is produced by our E.coli expression

system and the target gene encoding Cys24-Glu188(Lys46Arg) is expressed.

Accession#: P00030

Known as#: Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2

FORMULATION

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

SHIPPING

The product is shipped at ambient temperature.

Upon receipt, store it immediately at the temperature listed below.

STORAGE

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.

RECONSTITUTION

Reconstituted protein solution can be diluted with distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

QUALITY CONTROL

Mol Mass 19.4kDa AP Mol Mass 17kDa, reducing conditions.

Purity Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

BACKGROUND

At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.