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文献和实验Chromatin Assembly Using Drosophila Systems
chromatin structure and activity in vitro, it is essential to have a chromatin assembly system that will prepare extended nucleosome arrays with highly defined protein content that resemble bulk chromatin isolated from living cell nuclei in terms
Purification of Human Multiprotein Complexes using OneSTrEP Technology
; Viability and cell growth (i.e. using Trypan blue and cell counting). Protein extracts We use a protocol for making cytosolic and nuclear extracts from HeLa S3 cells grown on dishes that has been developed in the Almouzni
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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