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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
, Ku. Ku is composed of 70-kDa and approx 80-kDa subunits (called Ku70 and Ku80, respectively) and targets DNA-PKcs to ends of double-stranded DNA. DNA-PKcs is related by amino acid sequence to the phosphatidyl inositol kinase (PI-3 kinase) protein
to induce photochemical reactions that result in covalent linkages between the nucleic acid and the protein. The protein can be a purified or a recombinant RNA binding protein, but perhaps a more common application is the identification of proteins
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
, and so on. If the DNA molecule has promoter elements (to allow RNA polymerases to transcribe the DNA), one can also obtain usable amounts of pure RNA, and RNA with a coding region can be translated in vitro or in vivo to express a protein of interest. The PCR
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