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文献和实验Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector
of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections
Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus
Vaccinia virus (VV) has proven to be a very useful tool for the expression and analysis of foreign gene products. The most common method used to produce recombinant viruses involves the insertion of foreign genes into the thymidine kinase (TK
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
-ligate ), the antibiotic resistance gene will allow bacteria carrying empty vector plasmids to survive the selection.Transformation The reaction used to create recombinant DNA molecules (ligation reaction, etc.) is used to transform bacteria
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