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文献和实验Assays for RING Family Ubiquitin Ligases
classes of E3 ligases, the HECT domain and the RING finger-containing ligases. RING finger-based ubiquitination utilizes RING-containing protein subunits, or proteins with intrinsic RING domains, to catalyze the formation of polyubiquitin chains
Studies of the Ubiquitin Proteasome System
of E3 Enzymes Expressed in In Vitro Translation Systems Alternate Protocol 2: Determination of Ubiquitin Chain Variant Phenotype Basic Protocol 7: Chelation of Zinc from RING‐ and PHD‐Finger E3s Alternate Protocol 3: Inhibition of HECT Domain E3
Functional Assays on High‐Content Protein Microarrays
(SMI Radioactive) Assay on the ProtoArray Human Protein Array Basic Protocol 3: Kinase Substrate Identification (KSI) Assay on the ProtoArray Human Protein Array Basic Protocol 4: Ubiquitin/SUMO Ligase
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