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文献和实验Construction of Recombinant Human Cytomegalovirus
genome. These studies can extend to the introduction of viral or foreign genetic material into ectopic sites in the viral genome, to investigate viral cis -control sequences or to phenotypically modify or tag the recombinant virus. Genetically modified
Production and Characterization of Recombinant Human and Murine TNF
Here we describe the methods for the expression of human and murine soluble tumor necrosis factor (hTNF and mTNF) in Escherichia coli cells and for their extraction, purification, and characterization. The expression and purification procedure
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
to photo-isomerization than when bound to rhodopsin (3 ). In addition to RPE, the protein is expressed in Müller cells of the neural retina (4 ), ocular ciliary epithelium (5 , 6 ), and transiently in iris (7 ). Recent investigations show the protein
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