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文献和实验Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
peptide libraries are generated using an intein?based plasmid construct, and the cyclized sequence is randomized using a PCR procedure. By transforming plasmid libraries into host cells containing the two?hybrid fusions, cyclic peptide inhibitors
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
proteins) are relatively difficult to investigate by standard yeast two?hybrid methods either because they are retained at cellular membranes or they activate the system in the absence of a true protein interaction. The membrane?based yeast two?hybrid (MbY
High‐Throughput Screening for Protein‐Protein Interactions Using Yeast Two‐Hybrid Arrays
for parallel assays of large numbers of proteins in a single experiment. They provide an alternative to libraries for protein screening purposes. This unit describes a protein array composed of living yeast (Saccharomyces cerevisiae ) cells that can be used
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