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- 爱必信(Absin)
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- 2025年07月12日
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文献和实验Gene Knockout Techniques(1) The Knockout Process
centrifuge tubes. Set up library by distributing 50 worms per well to 1152 wells in 12-well untreated tissue culture plates containing low-EEO agarose and lawns of OP50 (96 12-well plates, numbered sequentially). We use Nichiryo Model 8100 repeat pipetters
systems usually only require a single protein,called Cas9, to perform the target cleavage. Recent years, thetype II CRISPR-Cas9 systems have been studied by several groupsin their native microbial domain to elucidate their molecular organizationsand
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
how the 6C technique can be incorporated with other techniques to discover all the chromatin regions in the nucleus that interact with a given gene or chromatin region of interest in a specific protein-dependent manner. Such information allows complete
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