NanoMolar Nickel / Cobalt Assa

ChIP assay: Chromatin immunoprecipitation assay was performed according to the protocol of ChIP assay kit

  1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells

常用试剂盒：BCA Protein Assay Kit

相关专题 在蛋白质定量试验中，最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒（BCA Protein Assay Kit）。因为BCA法蛋白定量具有很多优点，如1.实验操作十分简单方便;2.实验耗时短，快速;3.试剂灵敏度高，实验结果准确;4.所用的试剂稳定性好，而且经济实用，抗干扰能力强等。 实验原理： BCA(bicinchoninic acid)法蛋白浓度定量

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP