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- 2025年07月15日
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文献和实验Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
(3 ) and represents the second most abundant Ig in serum (4 ), can be purified by classical chromatographic approaches with an acceptable degree of purity. But several steps, such as ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration
Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA‐binding proteins using the biotin/streptavidin affinity technique. The protocol involves the following steps: (1) a biotinylated, labeled DNA fragment is prepared containing a binding site for the protein to be purified; (2) the biotin‐cellulose
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Reagents Ammonium sulfate (50% [w/v]) (optional, see Step 23) Antibodies, primary Affinity-purified primary antibodies are diluted in PBS containing 1% BSA. The dilutions must be adjusted for each individual primary antibody. Typical
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