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文献和实验Preparation of Intact, Highly Purified Phagosomes from Dictyostelium
phagocytes and is now commonly used to study host-pathogen interactions. Dictyostelium is genetically and biochemically tractable and is a most versatile experimental system. The classical protocol for purifying phagosomes formed by ingestion of latex beads
ELISA Procedure for Measuring Serum Antibody Titer
reader Pipette tips Solutions to Prepare for the ELISA ProcedureSodium carbonate (Na2CO3) buffer, 50 mM, pH 9.6 with 0.02% NaN3 PBS-T (PBS containing 0.05% Tween-20) 3% BSA in PBS-T Sodium acetate (NaOAc), 0.1 M ELISA Procedure Run duplicates
Immunofluorescence: Rabbit Anti-Murine RELMα
实验步骤 The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 15 days post-infection. The tip of the cecum was removed, rinsed in 1X PBS pH 7.2, and fixed in 4% PFA
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