- ￥10960
- Cedarlane
- CLAS11-1733
- 2025年07月11日
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999
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0.5 mg
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文献和实验was measured in front-face configuration. The measured fluorescence signal was a result of a specific interaction. To verify this, we performed the immunoassay using the same labeled antibodies, but with a nonspecific antigen, rabbit IgG
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
Immunoprecipitation of Proteins
in the lysis buffer with the Coomassie-based reagent. 9.Dilute the cell lysate to approximately 1mg/ml total cell protein with PBS to reduce the concentration of the detergents in the buffer.A more concentrated cell lysate (i.e.,10 mg/ml)may be necessary
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