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文献和实验Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli
to generate at least 10 viral gene products. Recent reports have indicated that there are extensive interactions between various HCV proteins: the core (capsid) protein can interact with itself (1 ) and with the El envelope protein (2 ); El protein
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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