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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
, the amount of enzyme should be increased. Problem: What can be done to improve ChIP when using chicken antisera that do not bind well to either protein A or protein G? [Step 47] Solution: We recommend adding 5 µg of a rabbit anti-chicken antiserum
免疫组化技术-Immunohistochemical Protocols
bovine serum albumine (BSA) instead of NGS. The solution should also contain 0.6% Triton X-100 to improve penetration into the tissue. One needs to decide in advance if the end result (final marker or tag) should be fluorescent or suitable
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