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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
). For a protocol to isolate and maintain mouse embryo fibroblasts (mEFs), see Preparing Mouse Embryo Fibroblasts (Nagy et al. 2006a ). For a protocol to derive or maintain ESCs, see De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts (Nagy et
inoculation of 3 ml of antibiotic containing media with the plasmid or cosmid-containing bacterial colony, and then increasing the culture volume to 50 ml, and then to 4 l. However, it was observed that recombinant cosmid DNA isolated from cell cultures grown
of GALT cells from an important fish species in the Mediterranean aquaculture, the gilthead seabream. Mechanical, chemical and enzymatic treatments were assayed. Nylon wool columns and continuous density gradients were used for further separation of cell
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