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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
). For a protocol to isolate and maintain mouse embryo fibroblasts (mEFs), see Preparing Mouse Embryo Fibroblasts (Nagy et al. 2006a ). For a protocol to derive or maintain ESCs, see De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts (Nagy et
Isolation of Dendritic Cells from Mouse Lymph Nodes
, density gradient separation produces a population of DC with a purity of 40-50%. Most contaminating cells are lymphocytes with a small fraction (usually less than 5%) of macrophages. The choice of lymph nodes is dependent on the purpose of the experiment
of the adjacent unit membranes interspersed by short regions of unit membrane separation of approximately 100-150 Å. In a zonula adherens , or "intermediate junction", a fine network of cytoplasmic filaments radiates from the cell membrane into the cytoplasm
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