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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Protein of GAL4 Activation Domain
- 形态:
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 保存条件:
-20°C/1 year
- 克隆性:
Polyclonal
- 保质期:
1年
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml
- 抗体英文名:
GAL4 Activation Domain rabbit pAb
- 规格:
50ul
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文献和实验an activation domain that either directly or indirectly interacts with the core cellular transcriptional machinery to increase transcriptional efficiency. Early work on transcriptional transactivators demonstrated the modular nature of these proteins by showing
A Guide to Yeast Two-Hybrid Experiments(酵母双杂交实验指南)
is carried out inside the nucleus of the yeast Saccharomyces cerevisiae, where the two proteins to be tested are expressed as hybrid molecules (Figure 1A). One is fused to the DNA-binding domain (BD) of a transcription factor such as Gal4 or LexA
Two-hybrid analysis of genetic
to the activation domain (here called the prey)are expressed in two different haploid yeast strains of opposite mating type (MATa and MATa),and the strains are mated to determine if the two proteins interact.Mating occurs when haploid yeast strains of opposite
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