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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
Fluorescein-NAD+
- 库存:
10
- 供应商:
TargetMol
- 规格:
81 μg
Product Introduction
Bioactivity
英文名:Fluorescein-NAD+
描述:Fluoroscein NAD+ is a substrate for ADP-ribosylation, providing a convenient non-isotopic alternative to radiolabelled NAD for use in PARP assays. Allows direct measurement of NAD-dependent enzymes, such as PARP, by fluorescence microscopy. This product is a replacement for R&D Systems product 4673-500-01 81 μg is supplied as 250 μl of a 0.25 mM solution in water.

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文献和实验系催化水解 NAD 的烟酰胺和核糖的糖苷键的酶,可生成烟酰胺和 ADP核糖, EC3. 2. 2. 5。存在于动物的脑、脾脏、链孢霉、细菌的这种酶亦作用于 NADP 和脱氨 NAD ,存在于红血球的酶仅作用于 NAD ,此酶可被烟酰胺抑制。
Labeling of Oligonucleotides with Fluorescein
Oligonucleotides are a convenient alternative to conventional, cloned DNA probes since they can be rapidly synthesized in large quantities relatively cheaply. They can be made to very specific regions of the genome, without reference
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
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