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文献和实验Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
v3.1 Cycle Sequencing Kit Protocol, Applied Biosystems 2002 (Part# 4337035A)The success of your sequencing reaction depends upon the quality and quantity of DNA template. The capillary system is very sensitive to contaminants such as proteins, RNA
Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
. 5.Decant the liquid using the quick centrifugation procedure described in step 3. Another 70% ethanol wash can be repeated prior to drying, but only is necessary if dye blobs are seen during the sequencing run. 6.Dry the pelleted reaction products loosely
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