H3-4基因敲除质粒

pLenti-H3-4-sgRNA (H3-4基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的H3-4基因敲除的质粒(L05740 pLenti-H3-4-sgRNA)、慢病毒(L05741 H3-4 Knockout Lentivirus)、HEK293T细胞(L05742 H3-4 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L05743 H3-4 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L05744 H3-4 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

H3-4基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	H3-4	8290	BC069079	NM_003493

 

About the gene
Official Symbol	H3-4
Previous Symbol	H3FT
Official Full Name	histone cluster 3 H3
Synonyms	H3t; H3/g; H3.4
Location	1q42.13
Gene Type	protein_coding
Uniprot ID	Q16695
Pathway/Library	Epigenetic Regulators Related Genes Library
Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.